User:Robertinventor/Simple animals could live in Martian brines - Extended Interview with planetary scientist Vlada Stamenković

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Excluded from search engines -- November 9, 2018 Planetary scientist Vlada Stamenković of the NASA Jet Propulsion Laboratory and colleagues have developed a new chemical model of how oxygen dissolves in Martian conditions, which raises the possibility of oxygen-rich brines; enough, the work suggests, to support simple animals such as sponges. The model was published in  on October 22. Wikinews caught up with him in an email interview to find out more about his team's research and their plans for the future. This is an expanded verson of the Wikinews article Simple animals could live in Martian brines: Wikinews interviews Vlada Stamenković which I collaborated on, with more background information in it (I'm the volunteer reporter who interviewed him).

Background information and intro
The  is far too thin for us to breathe, or indeed, for lungs like ours to extract oxygen at all. It has around 0.6% of the pressure of Earth's atmosphere, on average. This is mainly carbon dioxide; only 0.146% of that is oxygen. Yet the result of their modeling was clear. In the cold conditions on Mars these minute amounts of oxygen can get into the salty seeps of water which may be present there. What's more, the oxygen levels anywhere on Mars could reach the levels needed to support any microbial life that depends on oxygen.

Some organisms can survive without it, but oxygen permits a more energy-intensive metabolism. Almost all complex multicellular life on Earth depends on oxygen. They found that cold water would take up much more oxygen than warm water. In the coldest salty water in the polar regions, covering perhaps 6.5% of the Martian surface, oxygen levels on Mars may be get high enough for simple animals such as sponges.

As interviewed by Wikinews:
 * VS: Our work really opens up new possibilities for the Martian habitability, and that’s why it’s so exciting!

As previously interviewed by National Geographic (October 22):


 * Vlada Stamenković: We were absolutely flabbergasted. I went back to recalculate everything like five different times to make sure it's a real thing.



Some background may help before we get to the main interview with Vlada Stamenković.

(background information): You might wonder why they would focus their research on salty solutions. What about fresh water? It's because it is likely to be rare on present day Mars. Usually the air pressure is so low that fresh water is not stable even at just above freezing, at 0 °C. Mars does have a higher pressure atmosphere at its lowest points such as the depths of the huge ancient impact crater of the, and this does raise the boiling point of fresh water to 10 °C. However, that still means that it is close to boiling point already at 0 °C. If any ice melts, the water would evaporate away rapidly, indeed the pressure is so low that ice also isn't stable at that temperature.

However, salty brines can be liquid at well below 0 °C, for the same reason salt helps keep roads ice free. Salts, and very salty brines counteract the tendency of the water to evaporate at low pressures. They can also take in water from the atmosphere too, in the process known as deliquescence, and are especially good at doing this at low temperatures.

Curiosity discovered indirect evidence of deliquescence in the equatorial regions (through humidity measurements). These regions are so dry that there isn't even any ice in the surface soil, yet it found that brines form during winter nights in the top 15cm of the soil through. They take up water from the atmosphere at night when the salts reach temperatures of around -70 °C. This water then evaporates again as the soil warms up through the day, and the process repeats every day - night cycle.

There is indirect evidence for other salty brines on Mars, perhaps more habitable than the Curiosity brines. In their paper, Stamenković et al. mention the hydrated magnesium and calcium salts associated with the Recurring Slope Lineae. These seasonal streaks form in spring on sun facing slopes, extend and broaden through the summer and fade away in autumn. The streaks themselves are not damp patches, but they may be associated with thin seeps of brine just below the surface. Later research suggests dust flows may also be involved. However the hydrated perchlorate salts observation still has to be explained, as well as the seasonal timing, not correlated with the winds. This is considered to be good evidence that there is at least an element of seasonal hydration associated with the streaks. The literature on this topic has a vigorous dialog between researchers who favour greater or lesser elements of brines in this process.

Before these new results, scientists assumed any present day Martian life would be able to grow without oxygen. There are several possibilities for this, based on Mars simulation experiments. Martian life could include certain blue-green algae such as, some black fungi, and some purple salt loving found in salt ponds and hypersaline lakes on Earth. . Some lichens such as also have some potential for surviving in Mars surface conditions without oxygen. They can do this because the algal component is able to make the oxygen needed by its fungal component.

However oxygen rich brines would permit a more energy intensive metabolism and perhaps even true multicellular animal life such as simple sponges. Almost all complex multicellular life uses oxygen.

As previously interviewed by Scientific American (October 22):


 * VS: Our work is calling for a complete revision for how we think about the potential for life on Mars, and the work oxygen can do, implying that if life ever existed on Mars it might have been breathing oxygen

(background information): Stamenković et al in their paper cite research from 2014 that showed that some simple sponges can survive with only 0.002 per cubic meter (0.064 mg per liter). Some microbes that need oxygen can survive with as little as a millionth of a mole per cubic meter (0.000032 mg, or 32 nanograms per liter). In their model, they found that there can be enough oxygen for microbes throughout Mars, and enough for simple sponges in oases near the poles.

A historical 101 on multicellular life on Mars may be of interest here. First, of course back in the early twentieth century there was much speculation about multicellular life there, with Lowell even speculating that intelligent Martians built the canals that he thought he could see in his telescope. By the time of early spaceflight it was already clear that the atmosphere was far too thin for terrestrial animals, but there was some hope for plant life. But the early observations from space showed a barren crater covered land and since then the idea of life on Mars has focused mainly on anaerobic microbes and photosynthetic life.

In 2014, however, Vera et al surprised many astrobiologists with their experimental result that multicellular life could be possible on present day Mars. But only photosynthetic life able to produce its own oxygen. Some lichens, such as are able to survive in close to Mars-like conditions high up on Antarctic mountain ranges, and show promise in Mars simulation chamber experiments. They can do this because the algal component is able to make the oxygen needed by its fungal component. They can also do this without needing any extra water as intermediary, in the experiments they are able to grow in partial shade, using only the night time humidity of the atmosphere itself.

In principle you could also have microscopic (sub millimeter sized) multicellular animals in anoxic conditions. There are only a three species of such creatures known on Earth, however, and they are not candidates for life on Mars. They are three species of, tiny animals about the size of a large amoeba, are able to survive without oxygen in deep extremely salty mud sediments in the Mediterranean.

This new research greatly expands the possibilities for complex life on Mars. The paper includes a map of potential brine oxygen concentrations for calcium perchlorates brines on the surface of Mars (their figure 3a). These would be higher at the lowest points such as the floor of the, south of the equator, where the atmospheric pressure is highest, reaching around 1% of Earth's atmosphere and lowest of all in the mountainous tropical southern uplands. But the highest values of all would be for brines at the poles.

Stamenković et al found that even in the worst case they could devise, oxygen levels throughout Mars would be enough for the least demanding (oxygen using) microbes, at around 2.5 millionths of a mole per cubic meter (0.0008 mg per liter). That's the value for the tropical southern uplands, where temperatures are high and the atmosphere is thin, and for their brine with the lowest oxygen solubilities, sodium perchlorate. They also calculated this figure using their worst case estimate (where they do the calculations on their least optimistic assumptions). However, they give reasons for believing that their more optimistic best case calculations are close to the true situation.

The highest oxygen concentrations of all, occur when the water is colder, which is most easily attained in polar regions. They paid particular attention to two brines, magnesium and calcium perchlorates, common on Mars. In simulation experiments these stay liquid as they are to temperatures as low as -123 to -133 °C before they transition to a glassy state. They do this even when mixed with the soil of Mars (regolith). It's at these very low temperatures that the optimal oxygen concentrations can be reached.

For their calcium perchlorates map with, the lowest concentrations are around 25 millionths of a mole per cubic meter (0.008 mg per liter). For this brine regions poleward of about 67.5° to the north and about 72.5° to the south, could have oxygen concentrations high enough for simple sponges. Closer to the poles, concentrations could go higher, approaching levels typical of sea water on Earth, 0.2 moles per cubic meter (6.4 mg per liter), for calcium perchlorates. The brine that achieved the highest oxygen solubility is magnesium perchlorates. With this, oxygen concentrations could reach values as high as two moles per cubic meter (64 mg per liter) for the best case with supercooling.

On Earth, worms and clams that live in the muddy sea beds require 1 mg per liter, bottom feeders such as crabs and oysters 3 mg per liter, and spawning migratory fish 6 mg per liter, all well within their 0.2 moles per liter.. Saturated sea water is about 9 mg per liter at 20 °C ranging up to 11 mg per liter at 0 °C..

Interview
Wikinews asked him whether their research suggests potential for life as active as the Earth animals that can survive at similar oxygen levels.
 * Does your paper's value of up to 0.2 moles of oxygen per cubic meter, the same as Earth's sea water mean that there could potentially be life on Mars as active as our sea worms or even fish?
 * VS: Mars is such a different place than the Earth and we still need to do so much more work before we can even start to speculate.''

(background information): In their model, Oxygen gets into the brines at the poles so readily because they may reach extremely cold temperatures. These are far below the usual cold limit of life. It is not a hard limit because life gets slower and slower at lower temperatures to the point where individual microbes have lifetimes of millennia. Such life is hard to study. It's almost impossible to tell whether it is a) active and able to reproduce at those temperatures or b) active and not able to reproduce, or c) intermittently sometimes active and sometimes dormant. The reproduction can't be studied using cell counts. But the usual limit cited is -20 °C. That's well above the lowest temperatures studied in the paper which go down to -133 °C.

Dirk Schulze-Makuch has proposed that Martian life might evolve an exotic metabolism with the perchlorates of Mars taking the place of the salts inside the cells of Earth life. This would have advantages on Mars, with the brines inside their own cells acting as an anti-freeze to protect them against extreme cold. Also with their salts being so hygroscopic, they may help them scavenge water from the atmosphere and their surroundings.

With this background, Wikinews asked:
 * The temperatures for the highest levels of oxygen are really low -133 °C, so, is the idea that this oxygen would be retained when the brines warm up to more habitable temperatures during the day or seasonally? Or would the oxygen be lost as it warms up? Or - is the idea that it has to be some exotic biochemistry that works only at ultra low temperatures like Dirk Schulze-Makuch's life based on hydrogen peroxide and perchlorates internal to the cells as antifreeze?
 * VS: The options are both: first, cool oxygen-rich environments do not need to be habitats. They could be reservoirs packed with a necessary nutrient that can be accessed from a deeper and warmer region. Second, the major reason for limiting life at low temperature is ice nucleation, which would not occur in the type of brines that we study.

(background information): His first suggestion here is that the cool oxygen rich reservoirs could have warmer water come up through them from below. He doesn't say where the warm water would come from, but one possibility is from geological hot spots. Our orbiting spacecraft have not yet found any, but Olympus Mons has been active as recently as 2.5 million years ago. If sources of warmer water could rise to the surface from below and encounter these cold oxygen-rich brines, life could make use of oxygen where the two mix.

The other possibility is that microbes can continue to function at very low temperatures in the Martian conditions. After the interview I discovered that they go into this in section "3.2 The lower temperature limit for life and the potential of aerobic habitats" in the supplementary information.

From previous research, the brines he studies don't form ice crystals when cooled. Instead, they smoothly transition to a glassy state after supercooling. When microbes adapted to extremely cold conditions are cooled down, the interior doesn't freeze but transitions to a glassy state (Intracellular vitrification). This transition is driven by freezing of the external media. When living in calcium or magnesium perchlorates, with no external crystalization, they could continue to have liquid interiors enabling metabolic processes to continue right down to the supercooling limit of the brines. If the intercellular fluids can stay liquid, this will keep the viscosity low, and permit vigorous metabolic processes to continue. Even in vitrified cells, metabolic processes continue at a slow pace, but if the fluids stay liquid then fast metabolic processes are possible.



Their research also helps to explain the presence of some minerals on the Mars surface, such as manganese oxides which require conditions of water and oxygen to form. Some researchers hav suggested they formed in an early Mars atmosphere that was thick and oxygen rich (which doesn't require life; it could for instance be oxygen rich due to ionizing radiation splitting water). This new reseach shows that these minerals could also form without an oxygen rich atmosphere.

As previously interviewed by National Geographic (October 22):
 * VS: Our explanation doesn't need any special magic — it works on Mars today,

(background information): The idea that Mars had enough oxygen in the past for marine animals, billions of years ago, when the atmosphere was thicker, is not too surprising nowadays since the discovery of those manganese oxides. That it may have enough right now is what is so very surprising about this new research, given that it has such a thin atmosphere, with so little oxygen in it. The atmosphere is unbreathable, its trace amounts of oxygen can't be used by any form of terrestrial animal life, but the brines may be another story.

The paper is theoretical and is based on a simplified general circulation model of the Mars atmosphere - it ignores distinctions of seasons and the day / night cycle. But it takes account of topography (mountains, craters etc) and the axial tilt. They combined it with a chemical model of how oxygen would dissolve in the brines and used this to establish predicted oxygen levels in the brines at the various locations on Mars.

Wikinews asked if they have plans to look into a more detailed model:


 * and about whether there are any future plans for using a more detailed model with time variation diurnally or seasonally.
 * VS: Yes, we are now exploring the kinetics part and want to see what happens on shorter timescales.



(background information): Their model took account of the tilt of the Mars axis, which varies much more than for Earth (our axis is stabilized by the presence of the Moon). They found that for the last five million years conditions were particularly favorable for oxygen rich brines, and that it continues like this for ten million years into the future, as far as they ran the model. For the last twenty million years, as far back as they took their modeling, oases with enough oxygen for sponges are still possible.

Remarkably, as they say in the paper, present day Mars would have more oxygen available for life than early Earth had prior to 2.35 billion years ago. On Earth, photosynthesis seems to have come first, before complex multicellular life, generating the oxygen for the first animals. On Mars, with a different source for oxygen, oxygen breathers could arise before photosynthesis. They suggest in their paper that this gives broader opportunities for oxygen-breathing life on other planets.

Wikinews asked Vlada Stamenković if he had any ideas about whether and how sponges could survive through times when the tilt was higher and less oxygen would be available:


 * I notice from your figure 4 that there is enough oxygen for sponges only at tilts of about 45 degrees or less. Do you have any thoughts about how sponges could survive periods of time in the distant past when the Mars axial tilt exceeds 45 degrees, for instance, might there be subsurface oxygen rich oases in caves that recolonize the surface? Also what is the exact figure for the tilt at which oxygen levels sufficient for sponges become possible? (It looks like about 45 degrees from the figure but the paper doesn't seem to give a figure for this).
 * VS: 45 deg is approx. the correct degree. We were also tempted to speculate about this temporal driver but realized that we still know so little about the potential for life on Mars/principles of life that anything related to this question would be pure speculation, unfortunately.



(background information): When the Phoenix lander landed on Mars in 2008, what appeared to be droplets formed on its legs. They grew, coalesced, and then disappeared, presumably falling off its legs. It was not able to analyze these droplets, but simulations since then by Nilton Renno and his team using the Michigan Mars simulation chamber have shown that such droplets can form within minutes when salt overlays ice on Mars. With this background then Wikinews asked him if he had investigated the timescale, and if so, whether these brines could become oxygenated.


 * How quickly would the oxygen get into the brines - did you investigate the timescale?
 * VS: No, we did not yet study the dynamics. We first needed to show that the potential is there. We are now studying the timescales and processes.

(background information): It is no wonder that this is a challenge. For instance, Curiosity measures temperature changes of around 70 °C between day and night. Also there are large pressure differences between summer and winter. In Gale crater it varied from under 7.5 mbar to nearly 9.5 mbar. There are also large pressure differences between day and night, varying by 10% compared to a tenth of a percent on Earth. On Earth we see such large pressure differences only during a major hurricane.


 * Could the brines that Nilton Renno and his teams simulated forming on salt / ice interfaces within minutes in Mars simulation conditions get oxygenated in the process of formation? If not, how long would it take for them to get oxygenated to levels sufficient for microbes? For instance could the Phoenix leg droplets have taken up enough oxygen for aerobic respiration by microbes?
 * VS: Just like the answer above. Dynamics is still to be explored. (But this is a really good question 😉).

Wikinews also asked how their research is linked to the recent discovery of possible large subglacial lake 1.5 km below the Martian South Pole found through radar mapping.


 * Some news stories coupled your research with the subglacial lakes announcement earlier this year. Could the oxygen get through ice into layers of brines such as the possible subglacial lakes at a depth of 1.5 km?
 * VS: There are other ways to create oxygen. of water molecules into hydrogen and oxygen can liberate oxygen in the deep and that O2 could be dissolved in deep groundwater. The radiolytic power for this would come from radionuclides naturally contained in rocks, something we observe in diverse regions on Earth.

(background information): There's research by Möhlmann that suggests that fresh liquid water may form in the Martian polar region a few centimeters below clear ice, a process that happens regularly in Antarctica. If similar clear ice exists on Mars, this process should happen even at very low surface temperatures. Our reporter, referring to this research, asked him:


 * Could it get into a layer of fresh water just 30 cms below clear ice melted by the solid state greenhouse effect, as in Möhlmann's model (which forms subsurface liquid water at surface temperatures as low as -56 °C).
 * VS: See response above.

(background information): So, his answer here is that it could be possible by the same process, radiolysis of the ice through radioactivity in the rocks.

If there are indeed biologically friendly oases dotted throughout the surface of Mars then this could make it harder to sterilize spacecraft sufficiently to explore Mars. They have to be sterilized in order to avoid introducing Earth life to the habitats and so confusing the searches. If the surface of Mars has these oxygen rich habitable brines then it makes the sterilization requirements more stringent. As the Scientific American article suggests, it might be necessary to sterilize robots completely of all micro-organisms, which would drive up the cost of missions to Mars.

Stamenković as interviewed by Scientific American says


 * VS: I think there's a sweet spot where we can be curious and we can be explorers and not mess things up, We have to go for that.

(background information): NASA and ESA both have missions that they plan to launch to Mars in 2020 to search for life but both have the search for past life as their main focus. The last and only missions to search for present day "extant" life on Mars were the Viking 1 and 2 missions in the 1970s. Stamenković would like that to change.

As interviewed by Space.com (October 22) he said.


 * VS: There is still so much about the Martian habitability that we do not understand, and it's long overdue to send another mission that tackles the question of subsurface water and potential extant life on Mars, and looks for these signals

(background information): There are many such instruments we could send. One example, the "Chemical laptop" or PISCES under development at JPL is shown to the right. A National Academy of Sciences report released 10th October 2018 emphasizes the need to include in situ life detection instruments on future missions:

"The report highlights the need to include in situ detection of energy-starved or otherwise sparsely distributed life such as chemolithotrophic or rock-eating life. In particular, the report found that NASA should focus on research and exploration of possible life below the surface of a planet in light of recent advances that have demonstrated the breadth and diversity of life below Earth’s surface, the nature of fluids beneath the surface of Mars, and the likelihood of life-sustaining geological processes in planets and moons with subsurface oceans."

Vlada Stamenković is working on a new instrument TH2OR to send to Mars on some potential future mission. It would search for potentially habitable brines deep below its surface using ultra low frequency radio waves. This is a frequency far lower than that of ground penetrating radar, in the range of a fraction of a Hertz up to kilohertz. Wavelengths are measured in kilometers up to tens of thousands of kilometers or more. Wikinews asked him for more details


 * And I'd also like to know about your experiment you want to send to Mars to help with the search for these oxygenated brines
 * VS: We are now developing at “NASA/JPL-California Institute of Technology” a small tool, called TH2OR (Transmissive H2O Reconnaissance) that might one day fly with a yet-to-be-determined mission. It will use low frequency sounding techniques, capable of detecting groundwater at depths down to ideally a few km under the Martian surface, thanks to the high electric conductivity of only slightly salty water and Faraday’s law of induction. Most likely, such a small and affordable instrument could be placed stationary on the planet’s surface or be carried passively or actively on mobile surface assets; TH2OR might be also used in combination with existing orbiting assets to increase its sounding depth. Next to determining the depth of groundwater, we should also be able to estimate its salinity and indirectly its potential chemistry, which is critical information for astrobiology and ISRU (in situ resource utilization).



(background information): Wikinews asked if this device would use natural sources of ultra low frequency radio waves, or if it would use - a method that involves setting up a current in a loop to generate a sine wave and then suddenly switching it off and observing the radio waves generated by transient eddy currents. The eddy currents have been compared to a smoke ring, they propagate downwards and outwards, a circular current that gets wider as it gets deeper, creating secondary radio waves in a broad band including ultra low frequency waves. The Russian Mars 94 mission, canceled during the break up of USSR, would have flown a TDEM device to Mars.


 * Does your TH2OR use TDEM like the Mars 94 mission - and will it use natural ULF sources such as solar wind, diurnal variations in ionosphere heating and lightning?
 * VS: The physical principle it uses is the same and this has been used for groundwater detection on the Earth for many decades; it’s Faraday’s law of induction in media that are electrically conducting (as slightly saline water is). However, we will focus on creating our own signal as we do not know whether the EM fields needed for such measurements exist on Mars. However, we will also account for the possibility of already existing fields.

Technical details - guide to paper
Vlada Stamenković et al's paper explains that their work proceeded by modeling the physics of the solubility of oxygen in brines on Mars. Those were the mixes that permitted the lowest temperatures and so the highest oxygen concentrations. As salts are cooled down, any excess will come out of solution leaving a that has an optimal mixture of water with the salt (e.g. calcium perchlorate) to keep the brines liquid at the lowest possible temperature or eutectic point.

The brines can be cooled down below this theoretical lowest temperature without freezing, in a process known as supercooling. Experiments with Mars soil (regolith) simulants show that even with soil mixed in with the brines, they can still be supercooled to temperatures as low as -123 to -133 °C before transitioning to a glassy state.

They studied oxygen solubility with and without supercooling. They also studied two ways of modeling the physics, a best case, which matches the available data within a few percent, and a worst case simulation which gives a thermodynamic lower limit, however in their Methods section they say that their "best case" is most likely close to the actual situation on Mars. The main points in their research are summarized in their figure 3 which shows two versions of the map, with and without supercooling. The upper figure is the one with supercooling (note the colour-coding is different for the two maps). The map is for calcium perchlorates and they explain that results are comparable for magnesium perchlorates. The dotted lines in that diagram show the polar limit for sponges. The paper says that 6.5% of the surface area of Mars could have oxygen concentrations suitable for primitive sponges. The white and purple colored regions close to the poles are regions that could have oxygen solubilities similar to Earth's oceans, and the paper says that the polar regions have "the greatest potential to harbor near-surface fluids " at 0.2 moles per cubic meter of dissolved oxygen (6.4 mg / liter). The lowest concentration in their model for their best estimate with supercooling is ~2.5 × 10−5 moles per cubic meter of dissolved oxygen in the tropical southern highlands (0.0008 mg per liter).

For the effects of the different types of brine, see their figure 22. This is the one that covers the sodium perchlorate and magnesium perchlorate figures. The brines are colour coded as in figure 1, so sodium perchlorate is black, and Magnesium perchlorate is pink. The lowest number in the abstract of 2.5 millionths of a mole per cubic meter is for the sodium perchlorate black bar in figure 2e. The highest figure of 2 moles per cubic meter is for the magnesium perchnlorates pink bar in figure 2a.

The theory is covered in more detail in the supplementary information. In their figure S1, they start with a theoretical curve for liquid water at these ultra low temperatures - which achieves the maximum solubility if such was possible (of course in reality water would be frozen). The oxygen solubility is reduced by salting out factors which differ for the different salts. These are shown in their figure S2. Magnesium and calcium perchlorate have the two highest salting out factors but to compensate their brines remain liquid at far lower temperatures than potasium or sodium perchlorate. The upshot is that the highest oxygen concentrations can be achieved with magnesium perchlorate, with calcium perchlorate next.

The effects of the variations in the tilt of Mars' axis (obliquity) are summarized in their figure 4. Their figure 4a shows how the oxygen solubility varies with the tilt. The global maximum for supercooling and the best estimate is shown as a red line with purple dots and shows potential for sponges at angles up to around 45 degrees. Then figure 4c shows the effect of the variation in tilt for the last 20 million years and the next ten million years. The supercooling best estimate is shown at the top, the gray shaded boxes are times of atmospheric collapse. They observe in the paper that oxygen solubility levels have been particularly high for the last five million years and will continue in the same way for at least ten million years. They have been high enough for simple sponges for at least twenty million years. You can see how this works from this figure. The paper, discussing oases for simple sponges says that at present they are common poleward of about 67.5° north and about − 72.5° south.

They also make a connection with the recently discovered, and mysterious, seasonal variation in methane. This is about the variation in the background levels of methane, not the methane plumes observed from time to time by Curiosity. The background levels of methane observed by Curiosity over five years vary in a regular seasonal fashion from 0.24 to 0.65 ppbv reaching a maximum towards the end of the Northern hemisphere summer (Curiosity is a few degrees south of the equator at ). They suggest that at the solubilities they found in the paper, the oxygen could explain some surprising observations such as the highly oxidized phases in Martian rocks and this seasonable variability of methane in Gale crater.

The paper is available to read in its entirety through the link provided on the author's website and the Nature Sharedit sharing initiative.

Background information - why oxygen is so significant for multicellular life
There is much more energy available to an organism from the same amount of food with oxygen. Without oxygen, the that's the source of energy in Earth organisms can only be broken up into two  molecules (each of 3 carbon atoms). This process produces two molecules of, the energy molecule that powers our cells. This works quickly but the amount of energy is limited; it's the energy production method used in sprinter's "fast twitch" white muscle fibers.

With oxygen, glucose can be reduced all the way to carbon dioxide, and in the process can be produced from a single molecule of glucose. This is the type of energy production used in the "slow twitch" red muscle fibers of a, or runner. It lets them run for many miles using little by way of glucose from their food (about 100 calories of excess calories over the resting metabolic calories, depending on body mass, or around 25 grams of glucose per mile, which in turn is derived from and then fat as the race progresses). In this way a large organism can sustain itself with little by way of food if it relies on oxygen. Without oxygen a lifeform would need to eat more than ten times as much food to get the same amount of energy. It is hard for it to grow large because so much food is needed just to sustain itself and for reproduction. On Earth we don't know of any (organisms that can manage without oxygen) larger than those three species of, which are less than a millimeter in size. Anaerobes can buld large structures, as with the, which build up layer by layer but only the outermost layer, a thin biofilm, is actually alive.

What about extraterrestrial life though? Could it use something else in place of oxygen? Well, perhaps, but there is some earlier research that may suggest that it couldn't get very large. In 2005, Catling et al investigated the amount of energy available to carbon based organisms if with and without the use of oxygen in their metabolism. Oxygen produces the largest amount of free energy per electron transfer apart from fluorine and bromine, which are too reactive to build up in quantities useful to life. They found on general energetic principles that it still needs oxygen to sustain large complex lifeforms, from around 10 cms in size or larger. This is for any extraterrestrial food web for carbon based life.

They worked it out by looking at how many smaller organisms are needed to support larger ones in the food web. For aerobic life the number of organisms in a food web is inversely proportional to the mass, if you are ten times heavier then there are ten times fewer of you in the food web. These numbers are much less for.

According to Catling et al's modeling, if an organism is a hundred times larger (and so a million times more massive), then organisms of that size are a million times less numerous in an aerobic food web, but the same size of organism is a billion times less numerous in an anaerobic food web. So for instance, if you have a 200 micron organism preying on 20 micron organisms and they in turn on 2 micron organisms then there will be only a thousandth of the aerobic numbers in an anaerobic food chain. By the time you get to large organisms of 10 cm scale or larger they should be almost non existent in an anaerobic food web.

So, if Catling et al are correct in their inference here, then on general energetic principles that an extraterrestrial biosphere with large carbon based animals, at least of 10 cms scale or larger, is going to need oxygen. It seems to apply to Earth anyway. In anoxic environments, Earth animals have found it a challenge to get as large as 1 mm in size without oxygen, though it is possible that larger creatures lived on Earth when the seas were all anoxic.

TDEM
How it works and details of the Mars 94 TDEM, precursor to proposed TH2OR Mars 94 mission Proposal to use TDEM for Mars in situ resources same technology as for TH2OR

Brines and fresh water on Mars
Phoenix droplets Experimental work with the Michigan Mars simulation chamber reproducing the droplets: Possiibility for fresh water on Mars beneath ice / snow due to solid state greenhouse effect Big overview review paper on the research into possibilities for brines and fresh water on Mars Limits for water on Mars, close to boiling point: Curiosity's brines discovery - indirect but strong evidence Probable subglacial lakes near south pole.

Relevant Lifeforms proposed for Mars
Possibility of exotic biochemistry using perchlorates and hydrogen peroxide inside cells Lichens as first multicellular lifeforms proposed for Mars in modern times Black fungi for Mars (not multicellular)

Other background information
Example in situ life experiemnt that could be sent to Mars (one of many): Background to possibilities for life on Mars including discussion of cold limit. Methane on Mars: Variability of Martian temperature and pressure: Manganese dioxides, evidence of oxygen rich water on Mars in the past Evidence that it is still geologically active

CAT:SpaceCAT:MarsCAT:NASA CAT:BiologyCAT:Science and technology